GARRETT MARSHALL GOSS
A relentlessly-resourceful problem solver with a broad set of interests, an insatiable thirst for knowledge, and a passion for making.
University of Rochester, 2008–2012
Hajim School of Engineering and Applied Sciences
- B.S. cum laude with high distinction in Biomedical Engineering
- Cell & Tissue Engineering Concentration with supplemental Electrical and Chemical Engineering coursework, minor in Clinical Psychology
- Cumulative GPA: 3.7/4.0, Biomedical Engineering GPA: 3.7/4.0, Psychology GPA: 3.8/4.0
- Recipient of the Dean's Scholarship and the Samuel and Ruth Berger Endowed Scholarship
- Member of the Order of the Engineer and the Psi Chi International Psychology Honor Society
Mechanical Engineer - Manufacturing Integration, Oct 2019–Present
AURA Technologies, LLC
Quantitative Research Consultant, Aug 2018–Oct 2019
- Developed quantitative trading strategies on the Virtual Research Center platform.
Research Associate, Nov 2012–Aug 2019
Goldstein Lab, University of Miami School of Medicine Department of Otolaryngology
- As our lab's first hire, I played a significant role in transitioning our lab from a 2-person 'startup' to the 5+ member, NIH-funded lab that we have grown into. In this time, I generated a large body of work and laid the foundations for much of our future work, made significant contributions to a number of published journal articles and successful grant proposals, developed and troubleshot protocols for the majority of laboratory techniques that our lab uses for research each day, mentored a number of rotating students and fellow labmates, and served as a resource to our lab and to those around ours whenever there is a difficult problem to-be-solved, due to my demonstrated creative problem solving skills, outside engineering knowledge, broad knowledge-base, resourcefulness, and positive attitude.
- Investigated the role of olfactory neural epithelial stem cells in normal olfactory sensory development, tissue maintenance, lesion-induced regeneration, and age-related olfactory decline in human and rodent models.
- Developed the first rodent olfactory lesion model in which olfactory neurons do not reemerge after insult, using targeted diphtheria toxin fragment A induced ablation of olfactory progenitors during regeneration, to more closely mimic human anosmia (loss of sense of smell).
- Developed the first protocol to enable long-term culture and expansion of purified murine olfactory neural epithelial stem cells.
- Developed a custom, automated, end-to-end data processing pipeline for the annotation and analysis of video recordings from a behavioral test of mouse olfaction using Python. The pipeline performs all steps needed to go from raw captured video to publication-quality figures, including video processing, animal tracking, downstream analysis, and plotting. Reduced the time needed to analyze a single 10 minute video 240-fold, from 8 hours to 2 minutes. Also built the behavioral testing arena in which the testing is performed.
- Developed custom degenerate RNA PCR (polymerase chain reaction) primer sets to specifically recognize large fractions of the 1000+ olfactory receptor genes stochastically expressed in murine olfactory receptor neurons.
- Wrote software to automate the preprocessing (filtering, decimation, baseline-subtraction, and alignment) of electro-olfactogram (EOG) data traces collected in the smell-testing of anosmic or hyposmic mice following experimental treatment using MATLAB. Also built a large, low-cost Faraday cage to shield the electro-olfactogram recording setup from powerline (60Hz) and high-frequency (lighting ballast-induced, etc.) electrical noise, and a pipette puller (almost entirely from e-waste) to heat and pull glass micropipettes into recording electrodes of consistent (10 μm) tip diameter.
- Experience in performing a wide variety of laboratory techniques, including but not limited to rodent survival microsurgery and tissue microdissection, tissue processing and sectioning, immunohistochemical and immunocytochemical staining, mammalian cell culture, molecular biological assays at the DNA, RNA, and protein level (isolation and purification of each, primer design, PCR and qRT-PCR, electrophoresis, western-blotting, co-immunoprecipitation, in-situ hybridization, etc.), electrophysiology, calcium-imaging, microscopy (phase, DIC, epifluorescence, and confocal), and animal husbandry.
- Performed lineage tracing and conditional gene manipulations in transgenic mice using cre-loxP recombination. Maintained a large, actively breeding, multi-strain transgenic rodent colony.
Research Assistant, 2011–2012
Dickerson Lab, University of Rochester Medical Center Department of Neurobiology and Anatomy
- Programmed and troubleshot a PerkinElmer MultiPROBE II HT/EX robotic pipettor for the automation of high-throughput immunoassays.
Volunteer Research Assistant, May 2011–Aug 2011
University of Miami Miller School of Medicine
- Measured intracellular calcium transient and cardiomyocyte contractility using FURA-2, a dye sensitive to free intracellular calcium ions, and the IonOptix Myocyte Calcium and Contractility Recording System.
- Measured the superoxide scavenging potential of hydralazine, nitroglycerin, and the combination in freshly isolated murine ventricular myocytes using dihydroethidium (DHE), a dye sensitive to superoxide, and confocal microscopy.
Research Assistant, 2009–2011
Luebke Lab, University of Rochester Medical Center Department of Neurobiology and Anatomy
- Measured Acoustic Startle Response (ASR) and the effect of Prepulse Inhibition (PPI) on ASR in quiet and in the presence of masking noise in murine genetic mutants using a behavioral hearing-in-noise paradigm and a custom mechanical rig. Acquired and analyzed data using MATLAB and GraphPad Prism.
- Performed auditory brainstem response (ABR) testing of auditory function and Radio Frequency Identification (RFID) tag implantation survival surgery under anesthesia.
Solving Challenging and Important Problems; Product Design, Research, and Development from Concept to Production; Consumer Products; Additive and Subtractive Manufacturing; Robotics, Cobotics, and Bionics; Human-Centered Machine Learning; Intelligent Devices
- Rapid-prototyping of electro-mechanical systems
- 3D-printing (Cartesian, Delta), metalworking, woodworking, and machining (mill, lathe)
- Mechanical 3D Modeling (PTC Creo, OpenSCAD)
- Electronic design & EDA (KiCAD)
- Prototyping using off-the-shelf electronic development platforms and custom hardware, and a range of sensor and actuator modalities
- Python (Numpy, Pytorch, Jupyter, Tensorflow, OpenCV)
- Embedded C/C++ (primarily on Atmel and ARM microcontrollers)
- Desktop and embedded Linux (primarily on Ubuntu and Debian-based distributions like Armbian)
Selected Personal Projects
- Static Site Generator for garrettgoss.com: Beginning in 2019, I wrote Sitegen, an end-to-end static site generator in Python, for building and populating my personal site from blog-posts authored as Markdown-formatted text-files.
- FDM 3D Printer: in 2015, after having experimented with a low-cost kit-built 3D printer for several years, I built a large FDM (filament deposition modeling) 3D printer capable of printing objects up to 300mm x 300mm x 350mm in size and in a wide variety of thermoplastic materials. Of my own design, I built it by hand and largely from reused materials, and have made numerous tweaks and upgrades since.
- Mini Desktop CNC Milling Machine: in 2017, I designed and built a small, fully-enclosed CNC milling machine, also largely from recycled parts, enabling me to mill or engrave small and complex precision parts, under computer control, from materials unsuitable for use with standard at-home 3D printing, or that might otherwise might require strong acids or solvents to etch (e.g. prototype circuit boards).
- Robot/smart-device development platform (in progress): I am currently designing a modular development platform (custom hardware and software) for building internet-disconnected robots and smart devices. An on-board microprocessor, running a linux-based OS, will handle the execution of high-level code (written primarily in Python), while a microcontroller will be tasked with low-level and latency-critical functions (programmed using C/C++). Hardware modularity, versatility, and durability are continually emphasized in its design; thorough documentation and a diverse set of example applications will be built to showcase it. Designed using PTC Creo, OpenSCAD, and KiCAD.
For other examples, please visit my blog.
Academic Engineering Projects
- Surgical IV Fluid Administration in Adults: Measurement and Recording Without a Pump: Team-based research, design, and construction of a standalone biomedical device to noninvasively measure and log the administration of IV solutions in an operating room setting with minimal physician intervention. Working prototype delivered to the University of Rochester Medical Center Department of Anesthesiology for use in residency training. Business plan prepared for the device awarded third place at the 2012 Forbes Entrepreneurial Competition, hosted by the University of Rochester Simon School of Business.
- fMRI BOLD Analysis and Three Dimensional Visualization: Statistically analyzed a visual-cortical functional magnetic resonance imaging (fMRI) dataset to extract the blood-oxygenation-level dependent (BOLD) signal induced by the presentation of a patterned visual stimulus using MATLAB. Transformed the extracted BOLD signal into three dimensions for volumetric visualization of activation.
- Myoelectric Prosthesis Motor Control: Designed, simulated (OrCAD, PSpice, and MATLAB), and built an analog surface electromyography amplifier and motor controller using operational amplifiers.
- Durante M, Kurtenbach S, Sargi Z, Choi R, Harbour W, Goss GM, Kurtenbach S, Matsunami H, Goldstein BJ. Single cell analysis of olfactory neurogenesis and differentiation in adult humans. Nature Neuroscience. 2020 January 3. Manuscript Accepted.
- Kurtenbach S, Goss GM, Goncalves S, Hare JM, Chaudhari N, Goldstein BJ. Cell-based therapy restores olfactory function in an inducible model of hyposmia. Stem Cell Reports. 2019 May 1; DOI: 10.1016/j.stemcr.2019.05.001. PMID: 31155504.
- Goldstein BJ, Choi R, Goss GM. Multiple Polycomb epigenetic regulatory proteins are active in normal and regenerating adult olfactory epithelium. Laryngoscope Investig Otolaryngol. 2018 May 25; DOI: 10.1002/lio2.180. PMID: 30410986
- Kurtenbach S, Ding W, Goss GM, Hare JM, Goldstein BJ, Shehadeh LA. Differential expression of microRNAs among cell populations in the regenerating adult mouse olfactory epithelium. PLoS One. 2017 Nov 6; 12(11):e0187576. PMID: 29107942.
- Goldstein BJ, Goss GM, Choi R, Saur D, Seidler B, Hare JM, Chaudhari N. Contribution of Polycomb group proteins to olfactory basal stem cell self-renewal in a novel c-KIT+ culture model and in vivo. Development. 2016 Dec 1; 143(23):4394-4404. PMID: 27789621.
- Goss GM, Chaudhari N, Hare JM, Nwojo R, Seidler B, Saur D, Goldstein BJ. Differentiation potential of individual olfactory c-Kit+ progenitors determined via multicolor lineage tracing. Dev Neurobiol. 2016 Mar; 76(3):241-51. PMID: 26016700.
- Goldstein BJ, Goss GM, Hatzistergos KE, Rangel EB, Seidler B, Saur D, Hare JM. Adult c-Kit(+) progenitor cells are necessary for maintenance and regeneration of olfactory neurons. J Comp Neurol. 2015 Jan 1; 523(1):15-31. PMID: 25044230.
- Bas E, Van De Water TR, Lumbreras V, Rajguru S, Goss G, Hare JM, Goldstein BJ. Adult human nasal mesenchymal-like stem cells restore cochlear spiral ganglion neurons after experimental lesion. Stem Cells Dev. 2014 Mar 1; 23(5):502-14. PMID: 24172073.
- Dulce RA, Yiginer O, Gonzalez DR, Goss G, Feng N, Zheng M, Hare JM. Hydralazine and organic nitrates restore impaired excitation-contraction coupling by reducing calcium leak associated with nitroso-redox imbalance. J Biol Chem. 2013 Mar 1; 288(9):6522-33. PMID: 23319593.
- Finding the limits of what I know and pushing past them
- Developing elegant solutions to problems, technical or otherwise
- Prototyping, making, and remaking wonderful things from any available media:
- from wood, metal, plastic, textile, or food (woodworking, metalworking, machining, 3D printing, crafting, cooking),
- from that which already exists (repair, reverse-engineering, restoration),
- on screen, on paper, on canvas, or on film (digital graphics, code, mechanical or electrical CAD, painting, drawing, photography),
- for as little money, as much manual work (where there's more to do, there's more to learn), using as many unfamiliar techniques as possible